Effect of Temperature on Enzyme-catalyzed Reaction

Effect of Temperature on Enzyme-catalyzed ReactionYasmine DabashIntroduction case The denaturing of enzymes.Purpose To farther investigate catalysts and enzymes as well as their reactions to temperature change. caper How does temperature affect the prise of an enzyme-catalyzed reaction?ResearchSource 1 http//www.worthington-biochem.com/introbiochem/tempeffects.html reading found Like the majority of chemical reactions, the rate of an enzyme-catalyzed reaction raises as the temperature elevates. According to the source, A ten degree Centigrade rise in temperature depart increase the activity of most enzymes by 50 to 100%. some(prenominal) enzymes ar unfavorably affected by intense alter. Because most zoology enzymes quickly become denatured everywhere 40C, nearly whole enzyme activity is accomplished under that temperature. After some time, enzymes neutralize at even reasonable temperatures. The recommended temperature to store enzymes is 5C or lower berth but even some enzymes deactivate when frozen.Source 2 http//classroom.synonym.com/effects-temperature-enzyme-activity-biology-6049.html knowledge found Collisions mingled with molecules increase as temperature increases. This is because of the increase in pep pill and kinetic energy that is a result of increased temperature. With rapider velocities, there give be less(prenominal) time between molecule collisions. This results in secondary molecules reaching activation energy, which elevates the rate of the reactions. Since the molecules argon also moving quicker, collisions between enzymes and substrates also increase.Identify proteansIndependent Variable Liver temperatureDependent Variable The rate at which the colorful rises to the surface of the hydrogen peroxide.Controlled Variables Environment temperature, liver origin (same individual of the same species), concentration of hydrogen peroxide solution, weewee composition, interpenetrate radical size, filter paper weight and density, beake r material, forceps material, Petri dish material, filter paper material, and pipette material.Hypothesis If enzymes atomic number 18 het up(p) to 70C then the rate of enzyme-catalyzed reactions will decrease because the denaturing of enzymes leads by and by 40C.The political platformProcedure shade 1 Put on an apron, gloves, and safety goggles. Also, elucidate sure hair is tied up away from your face and come on of the way of any experimental material. maltreat 2 Use forceps to graze raw liver in an open Petri dish. Use a pipette to put a few drools of hydrogen peroxide solution on the liver. Observe any chemical or physical changes. (Caution Rinse away any hydrogen peroxide that comes in contact with skin, eyes, or clothing) tincture 3 Cut of a 5 cm x 5 cm theatrical role of filter paper and absorb it with a hear of liver.Step 4 Fill 50 a 50 mL beaker with 25 mL of 1% hydrogen peroxide solution using a graduated cylinder to measure.Step 5 Use forceps to drop the live red filter paper to the bottom of the solution. Record using a timepiece how long the filter paper takes to rise to the surface. Seek assistance if needed.Step 6 Now, heat 25 mL of water and a savour of liver in a 50 mL beaker to 70C with on a hot plate. (Caution Hot plates are called hot plates for a reason their hot. They tail assembly burn you. Seek adult assistance if spring chicken or incapable.)Step 7 Turn the hot plate take away and remove the beaker from the hot plate using beaker tongs. Then remove the liver from the water using forceps and set the sample into a Petri dish.Step 8 Repeat the previous experiment with the heated liver. Records results then analyze the data.Step 9 Compare and contrast the data sets from each part of the experiment. entropyLiver temperatureThe time it took for the liver to reach the surface of a 1% hydrogen peroxide. (seconds)Room Temperature Liver5.73 seconds70C Liver7.22 secondsResults and UnderstandingsGraphing info analysisWhen analyzin g the results of the experiment, it is easy to see that there are trends in the data. The human relationship between enzyme activity and temperature is inversely proportional. The higher(prenominal) the temperature of the enzymes, the faster they react and the less time the livered filter paper takes to rise to the surface of a 1% hydrogen peroxide solution. However, after 40C, the denaturing of enzymes therefore slowing lot enzyme-catalyzed reactions. This is because as the denaturing of enzymes pass alongs they no longer fit their specific lock to which they are key to.Though there was not any lack in accuracy, there was an extreme lack of precision during this experiment. Firstly, the assistant scientific pass on were not at all interested in the experiment qualification it exceedingly difficult to attempt to induce them on task, complete the experiment, and get steps that require the cooperation of more than one individual done. It is also greatness to recognize the limi tation of time allotted for previously mentioned tasks to be apply one class period. Also, it would have made all the difference in the world if the time it took for the filter paper to rise in increments of 5 mL could have been measured since it rose through 25 mL of 1% hydrogen peroxide solution. In doing this, it would be seen whether the filter paper accelerated positively or decelerated as it rose through the solution. unless due to time constraints, lack of expertise, and lack of an extremely precise measure device, such an idea could not be executed. But even in the beginning trying to accomplish that out of the ballpark idea, it would have been better to forgo more trials for each temperature of liver and perhaps entertain the addition of higher or lower temperatures of liver. Perhaps refrigerated liver? Even better, liver at or around 40C.Knowing how enzymes react to heat is vital for a number of biologists globally. Especially since biologists are required to aright h andle and store enzymes. Enzymes are best stored below 5C in order for them to not o dormant. Also, it is important for real manners biologists to understand that enzymes will work faster in heat further up to a certain point. For example, in many animals, enzymes denature after they hit 40C. Almost all enzyme activity happens below that temperature. terminationClaimIn correlation to the hypothesis, the outcome of the experiment proved the sideline hypothesis If enzymes are heated to 70C then the rate of enzyme-catalyzed reactions will decrease because the denaturing of enzymes occurs after 40C. The hypothesis gathered from the attest pertaining to the collision theory ended up being true not only on paper but in real animation experimentation and scenarios as well. Since the chemical reaction slowed when the livered filter paper was heated to 70C, then it is easy to say with utmost certainty that the relationship between temperature and reaction time is indirectly proportional up until 40C.In regards to the purpose of the lab experiment, many investigations have been made that further our knowledge on how to properly handle enzymes temperature wise. Which temperature are too hot, which are not hot enough, etc. Also, from the information gathered, other intelligent assumptions can be made on the proper storage of enzymes. Since it is known that enzymes, over time, become dormantwouldnt it be ideal to freeze them so they retain their activity? But perhaps, that is for yet another experiment.Evidence and exponentiationEvidence 1The collision theory states that as temperature rises, particles move more rigorously as collide more often. When these molecules bump into one another, reactions occurin this example, the enzyme-catalyzed reaction is the result. The amount of collisions it takes for a full reaction to occur is the activation energy of a chemical reaction. This theory is the first piece of evidence for the increase in enzyme-catalyst activity then t emperature increases.Evidence 2The bring forth Fit theory of enzymes accounts for the fact that the activity and substrate of an enzyme are complementary and mold to one anothers ice-cube tray, or as biologymad.com likes to say, Mould to each other like hand in glove. The link in substrates and enzymes bend when enzyme-substrate compounds form, deteriorating them significantly. Unlike the Lock and Key theory of enzymes, the Induced Fit theory accounts for catalytic action and the products of chemical reactions pertaining to enzymes.Works CitedEnzymes. biota Mad. Master Frameset, 07 Nov. 2005. Web. 07 Dec. 2014.Introduction to Enzymes Temperature Effects. Worthington Biochemistry. Worthington Biochemical Corporation, n.d. Web. 07 Dec. 2014.Santhosh, Lakshmi. The Effects of Temperature on Enzyme Activity. Synonym. Demand Media, 14 Aug. 2013. Web. 05 Dec. 2014.